Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

BACKGROUND Our study aimed to characterize and prospect immobilization strategies for a novel fungal peroxidase (POD) insert it in pollutant remediation context. The enzymatic extract was obtained by submerged fermentation of Trichoderma koningiopsis an alternative substrate consisting fresh microalgal biomass. efficiency evaluated monitoring the residual activity (RA) discoloration potential (DP) synthetic dye solution. Concomitantly, POD catalytic properties were explored, most promising storage strategy maintain studied. RESULTS non-purified guaiacol from T. expressed specific up 7801 U mg−1 free form, showing stability when subjected 80 °C pH range between 4.0 8.0. Furthermore, bioproduct immobilized on Fe3O4 magnetic nanoparticles, named nanozymes (MN-POD), 689% RA 100% removal Direct Brown 27 dye. An increase activity, both forms, also observed after 8 months. synthesized showed good reusability, maintaining 13 546 10 cycles, removing 94% color second batch. Toxicological evaluation with Allium cepa indicated that process essential eliminating genotoxic effects. CONCLUSION production presented this work are enzyme market wastewater treatment technologies due their high bioxidative potential. © 2022 Society Chemical Industry (SCI).